Relationship between antigenic diversity of Mycoplasma synoviae field strains and antibiotic susceptibility
3rd International Congress on Bacteriology and Infectious Diseases
August 04-06, 2015 Valencia, Spain

Boutheina Ben Abdelmoumen Mardassi, Awatef Béjaoui-Khiari, Amina Ben Allaya, Nabiha Bel Hadj, Béhija Mlik and Imen Chniba

Posters-Accepted Abstracts: J Bacteriol Parasitol


Mycoplasma synoviae is an important pathogen of both chickens and turkeys which causes synovitis, airsacculitis and egg
shell apex abnormalities in chickens and may result in significant economic losses in the poultry industry. Mycoplasma
synoviae has a great capacity to change its surface exposed antigens. Two major immunodominant and surface exposed
membrane proteins, MSPB and MSPA were proved to be antigenically variable and considerable variation in their size and
expression was reported within and among several strains of M. synoviae. This antigenic variation may contribute to the
inefficiency of sero diagnostic tools and therefore jeopardize control and antibiotic therapy programs. In this study we sought to
analyze the antigenic variability and its association with the antibiotic susceptibility of 23 Tunisian field isolates of Mycoplasma
synoviae. Antigenic variability was determined by Western blot and colony blotting using anti-sera directed against MSPA and
MSPB domains. All field strains were antigenically homogenous with the same antigenic pattern but significantly different from
that of M. synoviae reference strain. Enrofloxacin, doxycyclin, oxytetracyclin and aivlosin are among the most widely antibiotic
families used in poultry in Tunisia. In vitro susceptibility of the 23 Mycoplasma synoviae field isolates for these antibiotics was
determined by the micro broth dilution method. Resistance to enrofloxacin with MIC ≥32 μg/ml was demonstrated forall
isolates while a decrease of susceptibility to aivlosin for 50% of isolates was observed. This study argues for the predominance
in Tunisia of a single serotype of M. synoviae that is fully resistant to enrofloxacin and less sensitive to aivlosin.

Biography :

Boutheina Ben Abdelmoumen Mardassi is a Doctorate in Veterinary Medicine from National School of Veterinary Medicine at Sidi Thabet, Tunis-Tunisia. She has
completed her PhD from Biotechnology Research Institute at Montreal (BRI) and Montreal University in Canada. She was Post Doctorate from Armand-Frappier
Institute at Montreal, Quebec, Canada. Since 2000, she is a Permanent Researcher and a Head of Mycoplasmas Laboratory at Pasteur Institute of Tunis. She has
published more than 15 papers in avian and human scientific journals.