S B Kenenbayev, K M Bulatova, Sh Mazkirat and A T Raimbekova
Kazakh Scientific research Institute of Farming and Plant Growing, Kazakhstan
Posters & Accepted Abstracts: J Plant Pathol Microbiol
Significant part of wheat varieties has alien chromatin introgressions, including ryeâ??s genetic material. 1BL/1RS translocation has positive as well as negative selective traits. Varieties and biotypes of bread wheat with- and without 1BL/1RS translocation were differentiated by a complex of secalin bands in Ï?-zone, specific for rye. They were compared on total grain protein and its fractions content and qualitative characteristics of protein groups. Protein extraction was done according to TriboÑ?, E. et.al., (2003) resulted to separating albumins and globulins, amphiphilic proteins, gliadins and glutenins. According to spectra of high molecular weight glutenin subunits of all samples have Glu-1 quality score from 7 to 9. Genotypes without translocation were characterized by high intensity of bands with molecular mass 55.6 and 53.4 kDa, in the zone of low molecular weight glutenins and gliadins whereas samples with 1BL/1RS translocation have not such distinct components. Significant difference between genotypes without translocations on total seeds protein, amphiphilic and glutenin fractions content was not detected. At the same time wheat samples with 1BL/1RS translocation had higher content of amphiphilic proteins, which can be linked with the resistance of such forms to unfavorable stress factors of environment.
Email: serikkenenbayev@mail.ru