Ganesh Raj Pant
Scientific Tracks Abstracts: J Vaccines Vaccin
A trail batch of tissue culture inactivated anti rabies vaccine was produced at Rabies Vaccine production Laboratory in Kathmandu in 2010. Vero working cell line (164P) was used for propagation of cell that was infected with PV/RV at 100 MOI working rabies virus strain at 170 serial passages of cells. Virus cultures fl uid was harvested and pooled sample of fl uid was tested by using Anigen Rapid Test Kit for the detection of rabies virus. Pooled sample was found positive for rabies virus antigen where as the fl uid collected from control cell was negative for rabies virus. Th e harvested virus was then centrifuged at 10,000 rpm and condensed for purifi cation in pellicon machine (milli pore) under Pump Master fl ex speed 0.5, feed pressure 0.2 Kg/cm2 and pellicon fi lter membrane 0.02 um pore size. Th e titer of virus before inactivation was 10 7.5 TCID 50/0.1ml. Finally this virus fl uid was inactivated with beta propiolactone. Th e ED 50 of the titer of vaccine was 10 when challenged with CVS 50 LD50 /0.03 ml in NIH test. Th e potency (IU/ml) of this vaccine was 4.6 when tested in Nancy Wild Life Laboratory in France. Th e result of other quality test such as inactivation, toxicity, safety, specifi city, sterility and pH test were found satisfactory.
Dr. Ganesh Raj Pant is a Senior Veterinary Of fi cer and Chief of Rabies Vaccine Production Laboratory in Kathmandu within the Department of Livestock Services, Government of Nepal. Dr. Pant has been working in Nepal since 1983 and involving in the laboratory diagnosis as well as fi eld investigation of animal diseases. He has also been involved in the surveillance program of Rinderpest and Avian In fl uenza in his country. He passed his Bachelor degree in Veterinary Science from India and Master in Tropical Veterinary Science from UK. He is been in the research work and has published 15 research articles in national and international journals