Journal of Bacteriology & Parasitology

Prevalence of virulence and high level aminoglycoside resistant genes in Entrococcus faecalis strains

2^nd International Congress on Bacteriology & Infectious Diseases

November 17-19, 2014 DoubleTree by Hilton Hotel Chicago-North Shore, USA

Alireza Dolatyar Dehkharghani, Mohsen Imani and Mohsen ghelman

Accepted Abstracts: J Bacteriol Parasitol

Abstract:

Background: Enterococci have increasingly emerged as a cause of serious nosocomial and community-acquired infections, including bacteraemia and endocarditis. Enterococcus is capable of acquiring resistance genes, including high-level resistance (HLRA) to aminoglycoside antibiotics. It is believed that nosocomial enterococci might have virulence elements that increase their ability to colonize hospitalized patients. Objectives: The genetic and molecular basis of high-level aminoglycoside resistant (HLAR) and the prevalence of genes encoding virulence factors in high-level aminoglycoside resistant Entrococcus faecalis were investigated. Material and Methods: Bacterial identification was performed by biochemical tests and phenotypification was done. Antibiotic susceptibility was evaluated by disc diffusion and microdilution methods. Aminoglycoside resistance genes and virulence genes aceI, agg, gelE and efaA were analysed by PCR-based methods. Results: In this study, 24% of E. faecalis were high-level-resistant to gentamicin. The aac(6?)-Ie-aph(2??)-Iagene was responsible for high-level resistance to gentamicin in E. faecalis. The aph(3?)-IIIa and ant(4?)-Ia genes responsible for resistance to amikacin were also isolated in E. faecalis. The efaA gene was detected in 80% of the isolates. aceI gene was present in 55%, agg gene in 23.1%, and gelE gene in 46% of the clinical isolates. Conclusion: The spread of the aac(6?)-Ie-aph(2??)-Ia gene was responsible for high-level resistance aminglycoside among Enterococci isolated from patients. Virulence genes of E. faecalis also were isolated from clinical strains. We evaluated prevalence of virulence genes among isolated high-level resistant to aminoglycoside strains and understood efaA gene has the most frequency. More studies will be necessary to establish an association between two parameters.