Journal of Bacteriology & Parasitology

Polymerase chain reaction as an analytical tool in the diagnosis of cattle mastitis

International Congress on Bacteriology & Infectious Diseases

November 20-22, 2013 DoubleTree by Hilton Baltimore-BWI Airport, MD, USA

Daif H N, E AmryKh F, Khalil A, Marouf SA and El-Jakee J

Accepted Abstracts: J Bacteriol Parasitol

Abstract:

Mastitis is considered one of the most important dairy cattle diseases. Milk samples are susceptible to the contamination by pathogenic bacteria. These bacteria include Staphylococcus aureus, Listeria monocytogenes, Escherichia coli, Streptococcus agalactiae and, etc. Traditional methods for the detection of these pathogens are laborious and time consuming. So, rapid and accurate diagnostic methods are needed. In the present investigation, a total of 362 cows were examined for mastitis pathogens. The common isolates from the clinical mastitis cases were S. aureus (13.6%), E. coli (10.5%) and Streptococcus species (6.7%). Among the subclinical cases, E. coli was the most prevalent isolate (14.5%) followed by S. aureus (11.8%) and Streptococcus species (7.9%). By using multiplex PCR, followed by reverse transcription -PCR (RT- PCR), the aforementioned pathogens in mastitis milk samples could be detected. Compared to the conventional cultivation method and multiplex PCR method, RT- PCR approach has advantage to detect more bacterial strains.