Journal of Vaccines & Vaccination

Particulate systems-mediated DNA vaccines

12^th Asia Pacific Global Summit and Expo on Vaccines & Vaccination

November 24-25, 2016 Melbourne, Australia

Gulay Buyukkoroglu

Anadolu University, Turkey

Posters & Accepted Abstracts: J Vaccines Vaccin

Abstract:

Vaccines are expected to give lifetime protection from infectious diseases, ideally with a single immunization. An ideal vaccine should have the characteristics such as long-term immunity and efficacy with single dose and easy administration, simple manufacturing properties, resistance to temperature changes and multivalency. DNA based vaccines are among the latest developments in vaccine technology and consist of DNA plasmids containing the genetic sequence encoding desired antigens with the supporting transcriptional elements that ultimately allow efficient protein production. The advantages of DNA vaccines over conventional vaccines are the higher stability of pDNA, lower costs, decreasing the risks of infections due to weakened viral vaccines, ability to increase the multi-target antigen capacity on the plasmid and to generate humoral and cellular immune response. It has been observed in the study carried out that therapeutic immune responses are generated in many animal models as a result of the injection of the antigen coding DNA to the interior of the muscle. However, pDNA has low effectiveness due to the breakdown via enzymes after being administered to the muscle. That is why systems are needed that can protect the DNA from the effects of enzymes. In general, cationic and especially polymeric and lipidic transport systems have been used as DNA carrying systems for different administration routes such as oral, intramuscular, subcutaneous, vaginal and nasal. When compared with the breakdown of DNA as a result of the enzymes in the bodily fluids, it has been observed that the DNA encapsulated in particulate systems are better preserved than adsorbed naked DNA.

Biography :

Email: gbuyukko@anadolu.edu.tr