Journal of Vaccines & Vaccination

ORMOSIL nanoparticles as gene carriers: Synthesis, characterization and assessment of transfection effi ciency in cultured breast cancer cells

International Conference & Exhibition on Vaccines & Vaccination

22-24 Nov 2011 Philadelphia Airport Marriott, USA

Chandrababu Rejeeth, Soundrapandian Kannan, Shenbagamoorthy Sundarraj, Varatharaja Pradeepa, Maligamuthu Krishnan and Shanmugam Achiraman

Accepted Abstracts: J Vaccines Vaccin

Abstract:

Background Due to the ineff ective conventional treatment for breast carcinoma, the nonviral gene delivery system has been proved to be an attractive alternative to breast carcinoma therapy. Methods In this work, we have developed a kind of new self-assembled nanoparticles, which were named as organically modifi er silica nanoparticles (ORMOSILNs). Scanning electron microscopy and zeta potential results demonstrated that ORMOSILNs had a diameter of 40-80 nm and positive surface charges of +11.3 mV, respectively . Result ORMOSIL nanoparticles had been synthesized and incubated along with pCMV plasmid vector construct carrying p53 gene and transfected into the breast carcinoma cells. Th e confocal and electron microscopic studies further confi rmed that the nanoparticles were accumulated in the cytoplasm and the nucleus of the cancer cells transfected with p53 gene. Furthermore, ORMOSILNs could transfer foreign DNA into targeted cells with high transfection effi ciency and little cytotoxicity. Agarose gel electrophoresis revealed that the nanoparticles effi ciently complex with pCMV- p53 vector. Th e anti-cancer properties of p53 were demonstrated by assessing the cell survival and growth rate which showed a positive linear correlation in cancer cells. Conclusion Our results showed the ORMOSILNs, an effi cient gene vector, had the potential of gene therapy for breast carcinoma. Have a promising future for use as eff ective transfection agent for therapeutic manipulation of human cancer.