Journal of Bacteriology & Parasitology

Optimization of fermentation conditions for extracellular production of the antineoplastic enzyme, L-asparaginase by novel actinomycete Nocardiopsis synnemasporogenes sp. nov. NEAE-85

7^th World Congress on Microbiology

November 28-29, 2016 Valencia, Spain

Noura El-Ahmady Ali El-Naggar and Hassan Moawad

Genetic Engineering and Biotechnology Research Institute, Egypt
National Research Center, Egypt

Posters & Accepted Abstracts: J Bacteriol Parasitol

Abstract:

The optimization of different fermentation conditions for L-asparaginase production by Nocardiopsis sp. NEAE-85 and its validation using Plackett-Burman experimental design and response surface methodology was carried out. 15 nutritional variables (temperature, pH, incubation time, inoculum size, inoculum age, agitation speed, dextrose, starch, L-asparagine, KNO3, yeast extract, K2HPO4, MgSO4.7H2O, NaCl and FeSO4. 7H2O) were screened using Plackett-Burman experimental design. The most positive significant independent variables affecting enzyme production (inoculum age, dextrose and L-asparagine) were further optimized by the central composite face-centered design-response surface methodology. An overall about 3 and a half-fold increase in L-asparaginase production was achieved in the optimized medium as compared with the un-optimized basal medium. As a result, a medium of the following formula is the optimum for producing an extracellular L-asparaginase in the culture filtrate of Nocardiopsis synnemasporogenes sp. nov., NEAE-85: Dextrose 4 g, starch 20 g, L-asparagine 10 g, KNO3 1 g, yeast extract 1 g, K2HPO4 1 g, MgSO4.7H2O 0.5 g, NaCl 0.5 g, FeSO4.7H2O 0.01 g, pH 7, temperature 37 °C, agitation speed 100 rpm/min, inoculum size 4% ,v/v, inoculum age 24 h and fermentation period 5 days.

Biography :

Email: nouraelahmady@yahoo.com