Journal of Bacteriology & Parasitology

Optimization of biosorption conditions of hexavalent chromium by Aspergillus niger and Spicaria silvatica mycelial mats using taguchi orthogonal array

3^rd International Congress on Bacteriology and Infectious Diseases

August 04-06, 2015 Valencia, Spain

M A Ahmed1, T M A Abdel-Rahman1, N A Tharwat2 and A H Mostafa2

Posters-Accepted Abstracts: J Bacteriol Parasitol

Abstract:

In order to maximize the biosorption efficiency, eighteen experiments were designed to investigate the potentialities of
fungal biomass as a resistant and cost effective Cr+6 biosorbent. The influences of individual parameters as well as the interaction
between them were studied using Taguchi statistical design. Groups of statistical analysis that can define the relationships between
the responses of independent variables were carried out. In the current study, it was detected that the biosorbed Cr+6 by A. niger
and S. silvatica varied greatly among the 18 media used indicating the importance of optimization experiments in bioprocesses.
In case of A. niger the ANOVA analysis of variance revealed that temperature, mycelium status, NaNO3 and agitation were the
most significant factors influencing the Cr+6 biosorption. In S. silvatica, temperature, mycelium status, agitation and MgSO4
7H2O were the highly significant variables influencing Cr+6 biosorption. The design detected that the experimental values of
biosorbed Cr+6 were in reasonable agreement with the predicted values and the model was significant. The optimized predicted
medium from the software design for Cr+6 biosorption by A. niger and S. silvatica was dead mycelium, sucrose (15 gl-1), NaNO3
(1.5 gl-1), MgSO4 7H2O (0.1 gl-1), temperature (18ºC), pH (5), cultivation (static) and incubation period (5 days).