Journal of Microbial & Biochemical Technology

Nickel and vanadium toxicity in plant growth promoting microorganisms

Joint Event on 4^th World Congress and Expo on Applied Microbiology & 2^nd International Conference on Food Microbiology

November 29-December 01, 2017 Madrid, Spain

Grettel Aviles Sayas

Universidad Veracruzana, Mexico

Scientific Tracks Abstracts: J Microb Biochem Technol

Abstract:

The toxicity of nickel and vanadium, potentially toxic elements (PTE), in higher organisms has been widely documented because of its impact on crops or human health, however, toxicity in microorganisms has been poorly studied. The objective of this work is to determine the toxicity of nickel and vanadium in the growth of four strains of plant growth promoting microorganisms. Strains of Candida orthopsilosis B20, Serratia marcescens C753, Pseudomona tolasii P61 and Rhizobium tropici Ciat889 were inoculated into a minimal salt medium at pH of 6.5 at increasing concentrations of VOSO4 (0, 15, 30 and 45 mg �?� L-1) and NiSO4 (0, 25, 50 and 75 mg �?� L-1). They were shaken at 140 rpm at 28±2°C for 5 days. Their growth kinetics were measured by the turbidity method in a microplate spectrophotometer. Every 12 h, 200 μl of each sample was taken in triplicate and read at 600 nm, until 120 h. The data were processed by ANOVA, and Tukey's mean comparison test (α=0.05), with the SAS program for Windows 9.0. The growth of C. orthopsilosis B20 was severely affected by the presence of the two metals, R. tropici Ciat889 was highly sensitive to nickel in all concentrations, while in vanadium, in its lower concentration, it promoted its growth. Both strains of S. marcescens C753 and P. tolasii P61 showed tolerance to PTE and their growth was promoted in low concentrations.

Biography :

Grettel Aviles Sayas is currently a graduate student in Agricultural Science at the Universidad Veracruzana, Mexico. He has experience in the fields of biotechnology and microbiology, having worked in the Microbiology laboratorie of the Biological Research Institute of the Universidad Veracruzana, Xalapa, Mexico, in the department of in vitro culture of INBIOTECA, Xalapa, Mexico, and in the laboratory of Microbiology of the Colegio de Postgraduados, Texcoco, México. He worked in projects of genetic improvement in plants, and of degradation of hydrocarbons by bacteria. He did my degree in Biology (2015) and He is currently doing his Master's degree in Agricultural Sciences, both at the Universidad Veracruzana. At the moment he is working on the realization of two scientific articles.