Journal of Stem Cell Research & Therapy

Nanocarriers for Nose-To-Brain, Non-invasicve Delivery of Gene Therapy

7^th International Conference and Exhibition on Cell and Gene Therapy

March 15-16, 2018 | London, UK

Juan Sanchez-Ramos

University of South Florida, USA

Posters & Accepted Abstracts: J Stem Cell Res Ther

Abstract:

The overall objective of the present research was to develop a nanocarrier system for non-invasive delivery to brain of molecules useful for gene therapy. Manganese-containing nanoparticles (mNPs) carrying anti-eGFP siRNA were tested in cell cultures of eGFP-expressing cell line of mouse fibroblasts (NIH3T3). The optimal mNPs were then tested in vivo in mice. Following intranasal instillation, mNPs were visualized by 7T MRI throughout brain at 24 and 48 hrs. mNPs were effective in significantly reducing GFP mRNA expression in Tg GFP+ mice in olfactory bulb, striatum, hippocampus and cortex. Intranasal instillation of mNPS loaded with dsDNA encoding RFP also resulted in expression of the RFP in multiple brain regions. In conclusion, mNPs carrying siRNA, or dsDNA were capable of delivering the payload from nose to brain. This approach for delivery of gene therapies to humans, if successful, will have a significant impact on disease-modifying therapeutics of neurodegenerative diseases. jsramos@health.usf.edu