Maysaa Maysaa E. Zaki, Fathy Mansour, Mohamed Abou-Dobara and Basma Gaber
Accepted Abstracts: J Bacteriol Parasitol
Antibiotic resistance is a serious global public health problem. With increasing travel, transmission of drug-resistant organisms from one country to another became a possibility. Drug resistance is more frequently encountered in hospital-acquired pathogens; however the incidence of antibiotic-resistant pathogens in community acquired infections has been also on the rise in recent years. In the present study we have focused on the presence of multi drug resistant Staphylococci species associated with septicemia in neonate intensive care unit (NICU) in Mansoura University children hospital, Egypt and the outcome of infections by these species. Moreover we tested various isolates of Actinomycetes bacteria obtained from different localities of Egyptian farming soils to evaluate their antagonistic capacity against Staphylococci multi drug resistant species The study revealed the presence of 30 isolated Staphylococci species from NICU, 56.7% were isolated from early onset septicemia and 43.3% isolated from infants with late onset septicemia. The commonest species was Staphylococcus epidermidis (70%) followed by Staphylococcus aureus (S. aureus) (20%). Methicillin resistance was 90.9% and 95.7% among S. aureus and coagulase-negative Staphylococci respectively. Vancomycin resistance was 27.3% among S. aureus and 17% among coagulasenegative Staphylococci. There were the high attributable mortality rates among the neonates. Odds ratio for vancomycin-resistant Staphylococci indicates that cases with a laboratory-confirmed vancomycin-resistant Staphylococcus are 7.5 times more likely to die than those with other types of Gram-positive bacteremia. Testing the activity of isolated Actinomycetes from soil showed that five of the isolated actinomycetes (i.e. Streptomyces antimycoticus, Streptomyces malachiticus, Streptomyces rubiginosus, Streptomyces anulatus and Streptomyces rubiginosohelvolus). exhibited strong activities against multidrug resistant bacteria. Two isolates KS6, and KS21 inhibited the growth of all the tested bacteria. Future studies are needed to purify active compounds from these Streptomyces species and this could be the solution for extremely bad situation in multidrug resistant Staphylococci infections associated with life threatening infections.