Journal of Microbial & Biochemical Technology

Monitoring of antimicrobial resistance and genetic analysis of bacteria from meat and sea food in Korea

World Congress and Expo on Applied Microbiology

August 18-20, 2015 Frankfurt, Germany

Yonghoon Kim, Insun Joo, Eunjeong Heo, Soonhan Kim and Heejeong Lee

National Institute of Food and Drug Safety Evaluation, Korea

Posters-Accepted Abstracts: J Microb Biochem Technol

Abstract:

Distribution of antimicrobial resistant bacteria among agricultural, livestock and fishery products of domestic production and imported primary products in Korea was assessed. From the total 200 cases domestic food products analyzed in 2014, 77 E. coli, 55 S. aureus, 7 Enterococcus spp. and 9 Salmonella spp. were isolated. Imported 285 samples were analyzed and 29 E. coli, 13 S. aureus and 9 Enterococcus spp. were isolated. For E. coli, from domestic primary products, tetracycline, ampicillin, nalidixic acid resistance was relatively high compared to the other antimicrobials. And for imported products streptomycin, tetracycline, ampicillin resistance was relatively higher. In case of S. aureus penicillin resistance was higher in domestic products, but in imported products it was lower than results of previous studies. In case of Enterococcus spp. overall resistance decreased and continuous monitoring was required in future as number of isolated strains were so few. 2 MRSA strains were isolated from domestic beef and chicken meat and 2 ESBL producing E. coli from domestic chicken meat, 1 ESBL producing E. coli from imported chicken meat and 5 ESBL producing Salmonella spp. from domestic chicken meat were isolated. Tetracycline resistance of E. coli from domestic products that was slightly decreased from 57.9% (2013) to 51.9% in 2014 managed decreased level of resistance from resistance levels in 2006. Tetracycline resistance of E. coli from imported products showed lower resistance than that of previous year. In case of S. aureus, strains from domestic products had lower resistance than previous year and from imported products showed much lower resistance ever. PFGE and rep-PCR based comparative analysis was conducted against isolates of major resistant bacteria, which showed genetic similarity in 2 Salmonella spp. strains. Via MLST analysis, one MRSA strain from beef was determined as ST72 and one strain from chicken meat was ST692 and that was similar to precedent study in Korea.