Journal of Bioequivalence & Bioavailability

Molecular profile of the apoptosis-related genes in breast cancer cells after treatment with CpGoligodeoxynucleotides delivered by PAMAM-coated magnetic nanoparticles

2^nd International Conference & Expo on Biopharmaceutics and Biologic Drugs

September 14-16, 2016 San Antonio, USA

Negar Taghavi Pourianazar and Ufuk Gunduz

Middle East Technical University, Turkey

Posters & Accepted Abstracts: J Bioequiv Availab

Abstract:

CpG-oligodeoxynucleotide (CpG-ODN) is a potent stimulator of immune responses through triggering of a specific receptor called Toll-like receptor 9 (TLR9). In humans, TLR9 is expressed in numerous cells of the immune system and also in cancer cells. Our previous study indicated that activating of TLR9 by CpG-ODNs can generate a signal cascade for apoptosis in breast cancer cells. The purpose of this study was to examine the effects of CpG-ODN on the expression of the apoptosis-related genes in three different breast cancer cell lines, MDA-MB231, SKBR3, and MCF7. The expression profile of various apoptosis-related genes, Bax, Noxa, Bcl-2, Survivin, PUMA, and C-Flip, was studied and compared between untreated and CpG-loaded DcMNP-treated breast cancer cell lines. In evaluation of expression analysis of Bcl-2 and Bax, ratio of pro-apoptotic Bax to anti-apoptotic Bcl-2 expression was considered as an apoptotic parameter. Bcl-2/Bax ratio increased with CpG-loaded DcMNPs stimulation at the mRNA level in MDA-MB231 cells. Furthermore, a significant enhance in the amount of Noxa mRNA expression has detected in treated MDAMB231 and MCF7 cells which may contribute to apoptosis in these cell lines. The expression levels of C-Flip and Survivin mRNA were decreased significantly in treated SKBR3 and MDA-MB231 cells, respectively. In conclusion, the mechanism might be that CpGODN stimulates cell apoptosis through regulating the expression of apoptosis-related genes. DcMNPs is a targetable efficient delivery system for CpG-ODN which could be considered as a therapeutic agent in breast cancer.

Biography :

Email: negar_taghavi22@yahoo.com