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Molecular diversity of Cryptosporidium species from human cases in Scotland
2nd International Conference on Parasitology
August 01-03, 2016 Manchester, UK
Lisa Connelly, M Coyne, B L Jones and C L Alexander
Glasgow Royal Infirmary, UK
Posters & Accepted Abstracts: J Bacteriol Parasitol
Abstract:
Cryptosporidium, a coccidian parasite has been implicated in numerous waterborne and food-borne outbreaks associated with human and livestock disease. As part of outbreak investigations, the Scottish Parasite Diagnostic and Reference Laboratory, Glasgow (SPDRL) apply a glycoprotein (GP60) PCR/DNA sequencing-based approach to determine Cryptosporidium species/ subtypes from human isolates in Scotland. In 2014, 79 samples were speciated and shown to be C. parvum (n=48), C. hominis (n=29) and C. meleagridis (n=2). Eleven C. parvum isolates were sub-typed as part of outbreak investigations and consisted soley of the IIa family with 6 subtypes identified, namely IIaA13G1R2 (n=1), IIaA15G2R1 (n=2), IIaA16G2R1 (n=3), IIaA17G1R1 (n=3), IIaA18G2R1 (n=1) and IIaA21G4R1 (n=1). Eleven C. hominis isolates sub-typed as part of the same outbreak investigation were shown to be IbA10G2 (n=4) and IbA6G3 (n=7). The age of the infected patients ranged from <1-10 years (n=24), 11-20 (n=13), 21- 30 (n=20), 31->60 (n=22). More females (n=48) than males (n=31) were infected and only 4 cases had travel history stated. A peak of C. parvum cases occurred during April (n=14) and May (n=12), whereas most C. hominis cases occurred later in the year during October (n=5) and November (n=13). This update demonstrates the high degree of diversity of Cryptosporidium subtypes within Scotland.
Biography :
Lisa Connelly is a Biomedical Scientist at SPDRL, where she has been based since 2005. She has keen interest in research and development. Her recent publications include the molecular characterization of C. parvum isolates from human cryptosporidiosis cases in Scotland and Cryptosporidium species from human immunodeficiency-infected patients with chronic diarrhea in Jakarta, Indonesia.