Journal of Microbial & Biochemical Technology

Molecular detection of Helicobacter pylori among gastroduodenitis and peptic ulcer patients in Khartoum state

3^rd World Congress and Expo on Applied Microbiology

November 07-09, 2016 Dubai, UAE

Abbas Bakhit Mohammed Rahama

Al-Neelain University, Sudan

Posters & Accepted Abstracts: J Microb Biochem Technol

Abstract:

Helicobacter pylori infection is associated with gastroduodenitis, gastric ulcer and duodenal ulcer. Many of studies have released causes of gastric ulcer and duodenal ulcer (approximately 95% of duodenal ulcers and 85% of gastric ulcers) to infection with H. pylori. Helicobacter pylori (H. pylori), previously named Campylobacter pyloridis, is a Gram-negative, microaerophilic bacterium found in the stomach. H. pylori, a spirally shaped bacterium, 0.5-0.9 mM wide by 2-4 mM long. Like Campylobacter, it requires carbon dioxide for growth, but it has a tuft of sheathed unipolar flagella, unlike the unsheathed flagella of Campylobacter. H. pylori was identified in 1982 by Barry Marshall and Robin Warren, who found that it was present in patients with chronic gastritis and gastric ulcers, conditions that were not previously believed to have a microbial cause. It is also linked to the development of duodenal ulcers and stomach cancer. However, over 80% of individuals infected with the bacterium are asymptomatic and it has been postulated that it may play an important role in the natural stomach ecology. In 1893, the Italian researcher Giulio Bizzozero described helical shaped bacteria living in the acidic environment of the stomach of dogs. Helicobacter pylori, is the main cause of chronic active gastritis and has major role in development of duodenal ulcer, also associated with but not necessary the cause of gastric carcinoma ASM. There is 80% of chronic gastritis caused by H. Pylori. Since the discovery of Helicobacter pylori in the 1989 by Warren and Marshal, much has been learned about these Gram-negative spiral bacteria and its associated disease states. The study was aimed to detect Helicobacter pylori in patients with gastroduodenitis and peptic ulcer in Khartoum state by employing Polymerase Chain Reaction (PCR) to detect H. pylori 16S gene, Sudan. Molecular testing for H. pylori 16-S gene was done on 57 stomach and duodenal biopsy specimens using PCR technique. Biopsy specimens were collected by gastroenterologist using endoscopy. Multiple gastric biopsy specimens were taken from the stomach antrum and the corpus and the duodenum. Transport of specimens was in normal saline and kept at -80 oC till used. Extraction was done by using Vivantis GF-1 Nucleic acid extraction kit (Vivantis, Malaysia). The amplification reaction was carried out in thermo cycler machine PCR system with program system consisting of (1 min at 94 °C, 2 min at 55 °C, 3 min at 72 °C) and final extension was done at 72 °C for 5 min) PCR products were separated in a 1.5% agarose gel, then stained with ethidium bromide and viewed under gel documentation system. A result was considered positive when a band of the appropriate size was visible in the gel. Standard procedures for reducing contamination were strictly followed. Twelve samples (21.1%) out of 57 were positive by PCR, while 45 samples (78.9%) were negative. In conclusion the frequency of 16 S rRNA genes of H. pylori among endoscopic patients was 21.1%.

Biography :

Email: abbasrahama99@gmail.com