Journal of Bacteriology & Parasitology

Molecular, biochemical and immunological testing on the diagnosis of potential risk of food poisoning by coagulase negative Staphylococcus in products from animal origin

4^th International Congress on Bacteriology and Infectious Diseases

May 16-18, 2016 San Antonio, USA

Eduardo Mere Del Aguila, Nunes R S C and Paschoalin V M F

Universidade Federal do Rio de Janeiro, Brazil

Posters & Accepted Abstracts: J Bacteriol Parasitol

Abstract:

Food from animal origin, such as milk, dairy derivatives and meat can often be contaminated with Staphylococci-enterotoxigenic strains. Contamination may occur directly from infected animals, inadequate processing, like contamination of starter cultures or may result from poor hygiene during food manufacturing, retail and storage. Twenty-nine coagulase negative Staphylococci strains isolated from Minas frescal cheese and Italian type salami were identified by 16S rDNA sequencing: saprophyticus, xylosus, sciuri, carnosus, succinus, epidermidis, hominis and piscifermentans. A phylogenetic analysis grouped the strains into five major groups composed of 2 refined clusters containing sub clusters, where strains showed similarities over 90%. Enterotoxin encoding genes: sea, seb and sec showed a high prevalence in the genomes. The sei, seh, selm and seln genes were harbored by at least 33% of the strains. The tstH1 genes were found in 7% of the salami strains. Fifteen strains were able to transcript at least one type of mRNA enterotoxin, as evidenced by real time RT-PCR assays. In vitro production of the enterotoxin SEA-SEE was detected in almost all strains by ELISA assays. All strains showed multi-resistance to antimicrobials such as β-lactams, vancomycin and linezolid which show both human and veterinarian medicine therapeutic importance. MICs�?�0.06 mg/mL for methicillin, ampicillin and vancomycin and �?�0.25 mg/mL for linezolid were observed. High risk of food poisoning following the consumption of improperly manufactured animal origin products was emphasized, as well as the possibility of these food matrices acting as reservoirs for antibiotic resistance, spreading pathogenic strains.

Biography :

Email: emda@iq.ufrj.br