Journal of Blood Disorders & Transfusion

MiR-150 enhances immune tolerance by targeting ARRB-2/PDE4 to down-regulate inflammatory cytokines

6^th International Conference on Hematology

October 03-05, 2016 Orlando, USA

Dianzheng Zhang

Philadelphia College of Osteopathic Medicine, USA

Posters & Accepted Abstracts: J Blood Disord Transfus

Abstract:

MiR-150, a major modulator negatively regulating the development and differentiation of various immune cells is widely involved in orchestrating inflammation. In transplantation immunity, miR-150 can effectively induce immune tolerance, although the underlying mechanisms have not been fully elucidated. In the current study, we found that miR-150 is elevated after blocking CD28/ B7 co-stimulatory signaling pathway and impaired IL-2 production by targeting ARRB2. Further investigation suggested that miR- 150 not only repressed the level of ARRB2/PDE4 directly but also prevented AKT/ARRB2/PDE4 trimer recruitment into the lipid raft by inhibiting the activities of PI3K and AKT through the cAMP-PKA-Csk signaling pathway. This leads to the interruption of cAMP degradation and subsequently results in inhibition of the NF-kB pathway and reduced production of both IL-2 and TNF. In conclusion, our study demonstrated that miR-150 can effectively prevent CD28/B7 co-stimulatory signaling transduction, decrease production of inflammatory cytokines such as IL-2 and TNF and elicit the induction of immune tolerance. Therefore, miR-150 could become a novel potential therapeutic target in transplantation immunology.

Biography :

Email: dianzhengzh@pcom.edu