Journal of Bacteriology & Parasitology

Microbial load of operating theatre at Ayder Referral Hospital, northern Ethiopia

9^th Biotechnology Congress

August 31-September 02, 2015 Orlando,Florida, USA

Tewelde Tesfaye Gebremariam

Mekelle University, Ethiopia

Posters-Accepted Abstracts: J Biotechnol Biomater

Abstract:

Microbial contamination of the operating theatre (OT) is a major cause of nosocomial infections (NI). The study assessed the level of microbial contamination and to determine the antimicrobial resistance of the bacterial isolates. Settle plate�??s method was used for air sample collection while swab method was used to collect samples from surfaces and other articles in the major OT. Collected samples were transported and microbiologically processed using standard procedures. One hundred twenty air, 36 article and 12 surface samples were taken for microbiological evaluation. The highest level of microbial contamination was detected in the OT air before proper cleaning-fumigation as compared to after the intervention. Moreover, microbial growth was found on surfaces and semi-critical articles. On the other hand articles which were sterilized by autoclave showed no microbial growth. The five types of bacteria isolated were coagulase negative Staphyllococci (68; 53.4%), Staphyllococcus aureus (42; 33.1%), P. aeruginosa (13; 10.2%), E. coli (2; 1.6%) and Bacillus spp. (2; 1.6%). Methicillin resistance S. aureus (MRSA) account for 7.7% of the S. aureus isolates. The highest resistance was found against penicillin G and ampicillin with a resistance rate of 52.7% and 44.5% respectively. Multi-drug resistance was observed among 23(36.5%) of the bacterial isolates. In conclusion, there was high level of microbial contamination in the OT particularly in air and semi-critical articles. However, it has been dramatically reduced through proper cleaning-fumigation of the OT. Therefore, efforts should be made to ensure strict infection control practices in the OT.

Biography :

Email: ttesfayg@gmail.com