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Microbial comparative genome for in-silico DNA marker extraction
World Congress and Expo on Applied Microbiology
August 18-20, 2015 Frankfurt, Germany
Ali Najafi, Reza Ranjbar and Nadia Jahande
Baqiyatallah University of Medical Science, Iran
Posters-Accepted Abstracts: J Microb Biochem Technol
Abstract:
Next-Generation Sequencing (NGS) technologies have developed progressively in microbial genomic research and clinical applications. Also, genetic fingerprinting has been used in molecular epidemiologic studies. Therefore, it is essential that a connection or a link is established between them for discovering DNA marker in whole genome for using in molecular epidemiology and bacterial diagnosis. Bioinformatics and comparative genome analysis tools lead to further and deeper understanding of genomic variation in the bacterial species. Bacterial comparative genome analyses can be used for evolutionary processes, structure and function annotations, and importantly in unique DNA marker extraction. These unique probes are suitable for high-throughput diagnostic methods such as micro-array. Finally, setting up a procedure for comparative analysis will be useful for a wide range of microbial researches and clinical applications.