Journal of Bacteriology & Parasitology

Isolation and Characterization of Methicillin Resistant Staphylococcus aureus from Dog and Cat in Selective areas of Bangladesah

3^rd International Congress on Bacteriology and Infectious Diseases

August 04-06, 2015 Valencia, Spain

Marzia Rahman

Posters-Accepted Abstracts: J Bacteriol Parasitol

Abstract:

Methicillin-resistant Staphylococcus aureus (MRSA) is known to cause nosocomial infections and is now becoming
an emerging problem in veterinary medicine. The objective of the present study was to isolate and characterize the
methicillin resistant S. aureus (MRSA) fromdogs and cats of different veterinary hospital in Bangladesh as well as to study
the prevalence of MRSA in pet animals. A total of 108 samples consisting of nasal swab, pus and wound swab were collected
from dogs and cats. Initially S. aureus was isolated and identified on the basis of cultural, staining and biochemical properties
followed by molecular detection of S. aureus specific nuc gene. Subsequently methicillin-resistant Staphylococcus aureus
(MRSA)was detected phenotypically by disc diffusion test using oxacillin and genotypically by amplifying methicillin resistant
mecA gene. Out of 108 samples, S. aureus was isolated from 35 samples (32.40%) and MRSA was detected from four samples
(3.70%). All four MRSA isolates were detected from dogs (4.39%), while none of the examined cats yielded MRSA. Higher
prevalence of MRSA was recorded in CVH, Dhaka (5.12%) compare to SAQTVH, CVASU, Chittagong (4.87%). No MRSA was
found in VTH, BAU, Mymensingh. All MRSA isolates showed sensitive to vancomycin.This study suggests that dogs may act as
a potential reservoirs of MRSA from which human may get infection and thus spread MRSA in human community.