Journal of Bacteriology & Parasitology

In vivo antimalarial activities of extracts of Newbouldia laevis and Cocos nucifera against Plasmodium berghei

International Conference on Parasitology

August 24-26, 2015 Philadelphia, USA

Aderinola Adeyinka Aderonke1 and Ejiofor J I2

1Olabisi Onabanjo University, Nigeria 2Ahmadu Bello University, Nigeria

Posters-Accepted Abstracts: J Bacteriol Parasitol

Abstract:

Malaria caused by the parasite - P. falciparum is an acute disease which kills an estimated 863,000 people per year according to WHO report. Malaria chemotherapy failure is beginning to give medical practitioners a concern. The continuing spread of multi-drug resistant P. falciparum-malaria now poses a major threat to the tropics. Natural-products are the source of the two most important drugs currently available to treat severe falciparum-malaria, quinine and artemisinin derivatives. The development of these two important-drugs and utilization of many plants traditionally in various part of the world triggered the search for new, effective antimalarial drugs of natural origin. In this study, Swiss-albino mice ranging from 25-35 g of weight were used. The mice were randomly assigned into treatments and controls (negative and positive-control) with five mice per group. Plasmodium-berghei obtained from Nigeria Institute of Medical Research, Lagos were used as donors. Each mouse received 0.1 ml of diluted-blood containing 1x 106 P. berghei infected erythrocyte by intraperitoneal-route. Three hours after inoculation of the parasite, the mice in the three treatment groups received the extracts of Newbouldia laevis and Cocos nucifera in doses of 200, 400, 600 mg/kg orally for four consecutive days, while the negative and the positive control received normal-saline and 25 mg/kg chloroquine phosphate orally daily for four consecutive days. On the fifth day, blood sample was collected from tail snip of each mouse, thin smears were prepared, stained with 10%- Giemsa solution and examined under microscope with an oil immersion objective of 100x magnification power to evaluate percentage suppression. The extract treated mice (200, 400, 600 mg/kg) showed decreased parasitemia level to a highly significant level (p<0.05).

Biography :

Email: aderonkeaderinola@yahoo.com