Journal of Bioequivalence & Bioavailability

In vivo and in vitro antitrypansomal evaluation of crude methanolic extracts of Crotalaria albicaulis and Cistanche phelypaea against Trypanosoma evansi

Joint Event on 4^th International Conference on Drug Discovery, Designing Chemistry and Pharmaceutical Analysis & International Conference on Biobetters and Regulatory Affairs

June 27-28, 2018 | Vancouver, Canada

Yitagesu Tewabe

Addis Ababa University, Ethiopia

Posters & Accepted Abstracts: J Bioequiv Availab

Abstract:

African trypanosomiasis is a major disease of economic and public health importance affecting agricultural and human development. The search for alternative agents against African trypanosomiasis is justified by various limitations of existing chemotherapeutic agents. Thus, the aim of this study was to evaluate the in vivo and in vitro antitrypanosomal activity of the methanolic crude extracts of leaves of Crotalaria albicaulis and Cistanche phelypaea against Trypanosoma evansi isolate. The fresh leaves of both plants were extracted by percolation technique using methanol to obtain the crude extracts. For the in vivo assay, plant extracts at doses of 100, 200 and 400 mg/kg body weight were administered intraperitoneally daily for 7 days to mice infected with T. evansi. In vitro trypanocidal activity was performed in triplicate in 96 well micro titer plates. Diminazene aceturate and dimethyl sulfoxide were used as positive and negative controls, respectively. The in vitro study showed that the methanolic extract of C. albicaulis at 4.0mg/ mL concentration completely ceased parasite motility after 40 minutes. The plant also significantly (p<0.05) prolonged infection period during in vivo infectivity test. Moreover, at 400 mg/kg dose, C. albicaulis and C. phelypaea plants exhibited a mild in vivo antitrypanosomial activity against T. evansi. This study established that leaves of C. albicaulis and C. phelypaea have a mild in vivo and in vitro antitrypanosomal activity and can be considered as a potential source of new drugs for the treatment of camel trypanosomiasis.

Biography :

E-mail: yitagesu.tewabe@aau.edu.et