Journal of Bacteriology & Parasitology

Impact of putative bacteriocins against multidrug resistant clinical isolates

3^rd International Congress on Bacteriology and Infectious Diseases

August 04-06, 2015 Valencia, Spain

Samyyia Abrar1 and Saba Riaz1,2

Posters-Accepted Abstracts: J Bacteriol Parasitol

Abstract:

Background: Bacteriocins are well known for their antibacterial activity against multidrug resistant strains (MDRs). Lactobacilli
are known as friendly bacteria for their antibacterial activities against pathogens. The antibacterial activity of different strains
of Lactobacilli was analyzed against multidrug resistant strains (MDRs).
Objectives: Screening and characterization of multidrug resistant strains (MDRs). Characterization of the putative bacteriocins
produced by selected bacteria (Lactobacilli). Finally check the antibacterial effect of putative bacteriocins against multidrug
resistant strains (MDRs).
Materials and Methods: Multidrug resistant clinical isolates were selected on the basis of their MAR index. Well-Diffusion
assay was used for screening of putative bacteriocins produced by Lactobacillus strains against MDRs.
Results: Multidrug resistant strains were selected based on MAR (Multiple antibiotic resistance) index. Five bacteriocins
obtained from Lactobacillus strains isolated from commercial products. These bacteriocins showed a strong anti-bacterial
activity against selected MDRs. Decrease in zone sizes was observed when putative bacteriocins were treated with heat, SDS
and Protinase k. It was observed that Lactobacillus showed a significant antibacterial activity in-vitro in the presence of putative
bacteriocins against selected MDRs and further experiments are under process.
Conclusion: Putative bacteriocins produced by Lactobacilli exhibit significant antibacterial activity against MDRs. The
peptidal component of these bacteriocins can be used as an alternative therapy. Hence, it is necessary to purify the antibacterial
molecule out of putative bacteriocin for further analysis.