Jamal Hashemi
Tehran University of Medical Sciences, Iran
Posters & Accepted Abstracts: J Bacteriol Parasitol
Background: In recent years, the ubiquity of Candida infections by isolates resistant to common drugs and lack of proper diagnosis leads to failure in treatment. Considering the existence of the sensitivity pattern determinable for Candida isolates, to select the appropriate drug, it is essential to use the methods of determining the drug sensitivities of fungal agent. Therefore, we decided to study several anti-fungal drugs by using the disk diffusion and micro-dilution method against Candida species to consider. Methods: In this study, 48 clinical isolates of Candida were identified using various different tests such as germ tube, culture on CHROM agar candida, cornmeal agar-Tween 80 and PCR. Their susceptibility test on amphotericin B, Miconazole, econazole, fluconazole and caspofungin was undertaken according to the standard Disk Diffusion CLSI- M 44 A and CLSI M27A3 for microdilution. Results: The survey found that 98% of Candida studied was sensitive to amphotericin B, 85% were sensitive to Miconazole, 85% to econazole, 69% were sensitive to fluconazole and 100% of isolated were susceptible to caspofungin. No resistance to amphotericin B and caspofungin was observed and a few isolates were resistant to Miconazole, econazole and fluconazole respectively and some isolates showed dose dependent susceptibility. Conclusion: We observed that the Candida studied had the highest rates of sensitivity to caspofungin and amphotericin B. Among azoles the highest sensitivity respectively are to Miconazole, econazole and then to fluconazole. Candida albicans has the highest sensitivity to caspofungin and amphotericin B and also has a high sensitivity to fluconazole, Miconazole and econazole.