Journal of Pharmacovigilance

Hypothalamic cytokine as biomembrane stabilizer

6^th Pharmacovigilance Congress

September 28-30, 2016 Toronto, Canada

Petros Ghazaryan

Institute of Biochemistry-NAS, Armenia

Posters & Accepted Abstracts: J Pharmacovigil

Abstract:

Aim: The aim of the given work was to investigate the membranous aspects of the development of endogenous intoxication under radiation stress and the possibilities of its correction by studying the changes of the main lipid and protein components of biomembranes before and after introduction of proline-rich polipeptide-hypothalamic cytokine (HC). Materials & Methods: The test subjects were Wistar white male rats. The animals were exposed to ionizing irradiation. After 10 days of the exposure 3 Grey of HC was introduced intraperitoneally for 3 days at a rate of 5 mg per 100 g of animal�??s weight. Results: It is determined that the radiation stress is characterized by considerable metabolic derangements of the main classes of membranous phospholipids (PL) lymphocytes, thymocytes, and the cells of spleen tissue. Moreover, a double reduction of the activity of cytoplasmic glycerophosphate dehydrogenase and glycerol kinase is observed. At the same time a simultaneous, more than triple, increase of the activity of phospholipase A2 and of the processes of peroxide oxidation of lipids (POL) is observed. On the background of considerable (P<0.01) increase of the levels of lysophospholipids, phosphatidic acids and adenosine monophosphate, a reduction of the content of phosphatidylcholines and phosphatidylserines, as well as an inhibition of potency of Na/K- and Mg- ATP in spleen tissue and in blood lymphocytes is observed. After application of HC, a definite normalization of membranous phosphatidylserines and the activity of enzymes of phosphatidogenesis are observed. On the background of restoration of the content of adenosine monophosphates and ATP, the activity of ATP system, potency of phospholipase A2 and processes of POL is almost totally normalized. Possible mechanisms of development of endogenous intoxication under the radiation stress and its correction under the action of HC are being discussed.

Biography :

Email: ghazarpa@yahoo.com