Health Care : Current Reviews

HPTLC method for quantitative determination of quercetin in polyherbal formulation contains fractions of some hepatoprotective plants

3^rd Indo-Global Summit & Expo on Healthcare

October 05-07, 2015 New Delhi, India

Arti Gupta

Uka Tarsadia University, India

Posters-Accepted Abstracts: Health Care: Current Reviews

Abstract:

Tablet is poly herbal formulation made for liver protection. This formulation contains various 5 bioactive flavonoid fractions of 3 plant extracts from these 3 plants namely Butea monosperma, Ocimum gratissimum, Bauhinia vareigata contains quercetin flavonoid as a chemical constitute and it can be determined by HPTLC method. A sensitive and reliable high performance thin layer chromatographic method has been developed for quantitation of quercetin in bioactive flavonoid fractions. These bioactive flavonoid fractions chromatographed on silica gel 60 F254 plates with toluene: Ethyl acetate: Formic acid, 5: 4: 0.2 (v/v/v), as mobile phase. Detection and quantitation were performed by densitometric scanning at λ=380 nm, by using deuterium lamp. The accuracy of the method was checked by conducting recovery studies at three different levels, using the standard addition method and the average recovery of quercetin was found to be 98.8963%. The proposed HPTLC method provide a good resolution of quercetin from other constituents present in all plants extracts and can be used for quantitation of quercetin present in the all bioactive flavonoid fractions. The method is rapid, simple and precise.

Biography :

Email: aarti137@rediffmail.com