Journal of Blood Disorders & Transfusion

Hematopoietic stem cell transplantation for children with severe aplastic anemia: Single center experience

5^th World Hematologists Congress

August 18-19, 2016 London, UK

Wafaa Ezzat Ibrahim Mohamed, Matboly S, Adel Gawad A N, Abdel Gaffar A A, Talkhan H, Shalaby N A, Ezzelarab S A, Abdelaziz D and Mowafy N M

Ain Shams University, Egypt

Posters & Accepted Abstracts: J Blood Disord Transfus

Abstract:

Aplastic Anemia (AA) is a life threatening hematological disease that can be cured with hematopoietic stem cell transplantation. We have transplanted 13 children with severe AA from fully matched sibling donors between April 2011 and June 2014. They were followed up for a median duration of 3 years. All patients received ATG and Cytoxan conditioning regimen and PBSC.11 patients were engrafted. Neutrophil and platelet engraftment occurred after a median duration of 13 and 17 days respectively. Complete donor chimeras (>98%) in all patients; except one who showed partial chimera that followed with progressive decreasing chimera and rejection. Chronic GVHD (ocular) occurred in one patient 9 months post-transplant, CMV activation in four patients and skin infection in another two; All responded well to therapy. Immune reconstitution was studied by flow cytometry quantification of T and B-cell lymphocyte subsets and measurement of immunoglobulins IgM, IgG and IgA at 6, 12 and 18, and 24 months. NK (CD56+/16+) cells recovered in all patients by 6 months. Mean CD4 remained low in most of the patients up to 18 months and normalized in 3 patients at 2 years. CD19 B cell, CD3 T cell and CD8 T cell, as well as IgM and IgG immunoglobulins production were early and complete by one year in almost all patients. Young age, use of ATG and absence of chronic GVHD besides the presence of complete chimeras are responsible for the patients�?? good quality of life.

Biography :

Email: drwafaa_ibrahim@med.asu.edu.eg