Journal of Bacteriology & Parasitology

Genomic and proteomics approach of Acinetobacter baumannii MDR 107M, isolated from a hospital in Bogota, Colombia

International Congress on Bacteriology & Infectious Diseases

November 20-22, 2013 DoubleTree by Hilton Baltimore-BWI Airport, MD, USA

Quintero Carolina, Barreto Emiliano, Reguero Ma Teresa, Mantilla Ram?n, Valenzuela Emilia and Cepeda Alexandra

Posters: J Bacteriol Parasitol

Abstract:

Acinetobacter baumannii has become an opportunistic pathogen difficult for clinical treatment, because it has acquired resistance to diverse antibiotics. The presence of virulence factors in this microorganism has contributed to the rise of nosocomial infections. This research was focused on two aspects. First, identification of genes encoding factors associated with virulence in the genome of the multidrug-resistant strain A. baumannii 107m. Second, the study of outer membrane proteins (OMPs) associated with virulence of this bacterium. DNA were isolated, high-throughput sequencing by Illumina (HiSeq2000) and 454 Roche; genome assembly with mapping tools such as BWA and BOWTIE, and de novo assembly with Velvet and Newbler. Annotation was performed using NCBI Prokaryotic Genome Automatic Annotation Pipeline and also manual verification was done. In order to assess the expression of some genes coding for OMPs that might be involved in virulence, we proceeded to the isolation of OMPs and their analysis by 2D electrophoresis. We selected five spots with differential expression compared to that of A. baumannii ATCC 19606, which were identified by mass spectrometry. Proteomic results allowed the identification of OMPs associated with iron receptors, a protein associated with cell apoptosis and proteins involved in defense. The genomic data led to the identification of 103 genes involved in adherence, biosynthesis of siderophores and invasion. Genomic and proteomic results of this study, suggest that the multidrug-resistant A. baumannii 107m has genes involved in pathogenesis compared to A. baumannii ATCC 19606, however, more experimental studies are required to verify the A. baumannii 107m pathogenicity.

Biography :

Quintero Carolina is a biologist. During her Bachelor of Science at Pedagogical National University (Colombia), she achieved two scholarships as assistant student in biotechnology lab. Currently, she is a master?s student in microbiology at the National University of Colombia (Bogota Campus). There, she achieved a scholarship as outstanding graduated student. Since 2011, she belongs to the research groups of Molecular Epidemiology of Hospital Infection and Bioinformatics directed by Dr. Ram?n Mantilla and Dr. Emiliano Barreto respectively at the Institute of Biotechnology (IBUN). She participated in the 12th International Congress of National College of Bacteriology in 2012, which took place in Cali-Colombia.