Journal of Microbial & Biochemical Technology

Genetic analysis of Crimean Congo hemorrhagic fever virus in Iran

3^rd World Congress and Expo on Applied Microbiology

November 07-09, 2016 Dubai, UAE

Nariman Shahhosseini and Sadegh Chinikar

Bernhard Nocht Institute for Tropical Medicine, Germany
Pasteur Institute of Iran, Iran

Posters & Accepted Abstracts: J Microb Biochem Technol

Abstract:

Background: Crimean Congo Hemorrhagic Fever (CCHF) caused by CCHF virus, belongs to Nairovirus genus and Bunyaviridae family. The route of transmission is through the bite of infected ticks, handling of infected blood or organs of livestock and nosocomially. The virus has a negative and three segments (S, M and L) RNA genome. The initial phylogeny study on CCHFV in Iran showed that isolated Iranian strain (ArTeh193-3) was similar to Senegalese strain. Methods: The CCHFV-RNA was extracted from human sera and tick samples collected from different parts of Iran during 15 years. The virus genome was examined by RT-PCR. Positive samples were sequenced and sequences were analyzed by Geneious. Results: Phylogenetic investigations on CCHFV sequences obtained from human and tick samples showed that clade IV (Asia-1 and 2), clade V (Europe) and clade VI (Greece) are circulating genomic variants of CCHFV in Iran. Conclusion: Interestingly, all phylogeny studies during the last 15 years indicated that CCHFV strain Iran/ArTec 193-3 is likely fade away from Iran and it has been never reported since 35 years ago when it was reported as the first CCHFV strain from Iran. Since understanding the molecular epidemiology of CCHFV is the sine qua non to develop and implement surveillance strategies for its control, phylogenetic analysis of CCHFV is worthy of note. As endemic countries for CCHF surround Iran, high genetic diversity for CCHFV could be expected.

Biography :

Email: nariman.shahhosseini@yahoo.com