Awards Nomination 20+ Million Readerbase
PMC/PubMed Indexed Articles

Binding of Factor VIII to Lipid Nanodiscs Increases its Clotting Function in a Mouse Model of Hemophilia A

Google Scholar citation report
Citations : 1851

Journal of Blood Disorders & Transfusion received 1851 citations as per Google Scholar report

Flyer image
Journal of Blood Disorders & Transfusion peer review process verified at publons
Flyer image
Indexed In
  • Open J Gate
  • Genamics JournalSeek
  • JournalTOCs
  • Ulrich's Periodicals Directory
  • RefSeek
  • Hamdard University
  • EBSCO A-Z
  • OCLC- WorldCat
  • Proquest Summons
  • Publons
  • Geneva Foundation for Medical Education and Research
  • Euro Pub
  • Google Scholar
View More
Useful Links
Share This Page
Journal Flyer
Flyer image
Open Access Journals
View More
Gene expression of P-selectin in malaria infection
3rd International Conference on Hematology & Blood Disorders
November 02-04, 2015 Atlanta, USA

Okorie Hope Mery, Ohalete C N, Dike Ndudim J N, Edward Ukamaka, Egbuobi R C and Nwosu D C

Imo State University, Nigeria

Scientific Tracks Abstracts: J Blood Disord Transfus

Abstract:

Platelet selectin (P-selectin or CD62P) is an intercellular adhesion molecule with a strategic role in malaria. Homogenous sampling of subjects with normal hemoglobin (Hb AA), Sickle Cell Trait (Hb AS) and Sickle Cell Anemia (Hb SS) subjects was carried out in the ratio of 50:30:20 and by real time PCR, the normalized transcript level of P-selectin (SELP-ncn) were measured in malaria infections compared to non-infection control group within the sampled population. I also established malaria infection by standard PCR method, blood film microscopy and rapid diagnostic testing (RDT). Hemoglobin levels and age distribution of the participants across the different genotype groups were analyzed. The results show that the ABI-1 quantity passed normality test (Kruskal Wallis test, P=0.0403) but did not differ significantly when compared across groups indicating good sample quality. SELP-ncn showed no significant difference across all Hb genotypes studied (Kruskal Wallis test, P=0.2796) suggesting that P-selectin is expressed in both normal and sickle hemoglobin. Though the SELP-ncn did not differ significantly when compared across the groups studied, targeting it may help reduce the complications of malaria. RDT showed moderate sensitivity (41%; 95% CI: 21%-64%) and a high specificity (98%; 95% CI: 89.7%-99.9%). Blood film microscopy had a moderate sensitivity (68%; 95% CI: 52%-81%) and specificity (90.3%; 95% CI: 74%-97.6%) when PCR was considered as the gold standard. Median Hb concentrations of M-AA and M-SS; M-AA and NMSS; M-AS and M-SS, M-AS and NM-SS; M-SS and NM-AA; M-SS and NM-AS; NM-AA and NM-SS; NM-AS and NM-SS differed significantly (Kruskal Wallis test, P=0.0001). Median age of participants across groups differed significantly between M-AA and M-SS; M-AA and NM-SS (Kruskal Wallis test, P=0.0020).

Biography :

Email: hope4okorie@yahoo.com

PDF HTML