GC-MS, AAS, antioxidant and antiplasmodial activities of methanolic leaf extract of Morinda lucida (Ewe Oruwo) in male swiss mice infected with Plasmodium berghei NK65
5th International Conference on PARASITOLOGY & MICROBIOLOGY
July 12-13, 2018 Paris, France

Momoh Johnson Oshiobugie, Damazio Olanrewaju Anthony and Ajetunmobi Asibiallau Oladipupo

Lagos State Polytechnic, Nigeria

Posters & Accepted Abstracts: J Bacteriol Parasitol

Abstract:

Medicinal plants have been used for the treatment of many infections and diseases including malaria. The study was conducted to determine the effect of In-vivo anti-plasmodial and antioxidant properties of methanolic leaf extract of Morinda lucida in swiss albino mice infected with Plasmodium berghei NK65. The GC-MS result of the Morinda lucida shows the presence of five bioactive compounds. It was also observed that the plant contains the following minerals: copper, phosphorus, potassium, magnesium and iron. The phytochemicals and antioxidant property of the plant was also determined. Swiss albino mice were inoculated intraperitoneally with Plasmodium berghei NK65. Thirty-five mice were grouped into seven groups, five per group. Group A were not infected with P. berghei. Group B, C and D served as the negative and positive control groups while Group E, F and G were treated with 400, 600 and 800 mg/kg body weight of methanolic leaf extract of Morinda lucida. The extract caused 30.96%, 32.93% and 67.24% reduction in parasitemia at 400, 600 and 800 mg/kg body weight respectively while chloroquine exerted 96.53% and artesunate exerted 92.03% reduction at 10 mg/kg body weight respectively. The hematological parameters showed that the extract is not hematotoxic since it significantly (P<0.05) reduced WBC count, and increase RBC, HGB, and HCT values in the treated mice compared to the infected untreated mice. The histopathological study shows that the plant reduces the damage caused by the parasite. This study shows that the mean lipid peroxidation (MDA) level was significantly decreased in the malaria treated groups (Group C, D, E, F and G) compared to the negative control group. There was also a significant increase in the total protein, catalase, reduced glutathione, SOD% inhibition, SOD unit and Vitamin A levels in the liver homogenate of animals treated with chloroquine, artesunate and extract of Morinda lucida compared to that of the negative control group. The study shows that Morinda lucida possess antiplasmodial activity.