Journal of Blood Disorders & Transfusion

Fetal hemoglobin gene expression in patient with sickle cell disease attending university of abuja teaching hospital

3^rd International Conference on Hematology & Blood Disorders

November 02-04, 2015 Atlanta, USA

Amos Dangana

University of Abuja Teaching Hospital Abuja, Nigeria

Posters-Accepted Abstracts: J Blood Disord Transfus

Abstract:

Fetal hemoglobin (HbF) plays a dominant role in ameliorating morbidity and mortality of hemoglobinopathies. We evaluated the effects of polymorphic markers within the β-globin gene cluster to identify the genetic mechanics that influence HbF on sickle cell patients (n=242). Haplotype analysis was carried out by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and the framework polymorphism was established by PCR-sequencing, four independent regions of interest were identified: The 5�?? region of β-LCR-HS2 site, the intervening sequence II (IVSII) region of two fetal (Gγ and Aγ) genes and the 5�?? region of β-Globin gene. The correlation of these various haplotypes and SNPs with HbF expression and clinical data was studied. Our data suggest strongly that there is a significant association between markers of the β-globin gene cluster and HbF levels in SCD patients attending university of Abuja Teaching Hospital, Abuja. In this study, we showed that among the various polymorphic markers analyzed, only the sequence (AT)xN12(AT)y in LCR HS2 region was significantly associated (p<0.05) with increased HbF levels, suggesting that the β-globin gene cluster exerts a significant effect on HbF in sickle cell patients. This study can improve understanding of the pathophysiology of the disease and aid to increase our ability to predict clinical severity.

Biography :

Email: isalemit@yahoo.co.uk