Journal of Microbial & Biochemical Technology

Fecal carriage of ESBL-producing Escherichia coli among the migratory bird white stork (Ciconia ciconia), Algeria

Joint Event on 4^th World Congress and Expo on Applied Microbiology & 2^nd International Conference on Food Microbiology

November 29-December 01, 2017 Madrid, Spain

Esma Bendjama, Amira Bouaziz, Lotfi Loucif, Ammar Ayachi and Jean Marc Rolain

University of Batna2, Algeria
Annaba University, Algeria
Aix-Marseille University, France

Posters & Accepted Abstracts: J Microb Biochem Technol

Abstract:

The role of migratory birds in the trans-boundary dissemination of drug resistant bacteria including extended spectrum β-lactamase (ESBL)-producing Enterobacteriaceae is usually misjudged. The aim of this study was to screen for the presence of ESBL-producing Escherichia coli in white stork (Ciconia ciconia) migratory bird stools, then to investigate their molecular support of β-lactamase-production. In May 2014, eleven fecal samples of white stork (Ciconia ciconia) were collected in the Commune of El Madher in Batna, Algeria. Samples were subjected to selective isolation of third generation cephelosporins resistant Enterobacteriaceae. On the basis of characteristic pink-colored colonies on MacConkey agar, representative colonies were identified by biochemical testing and confirmed by matrix-assisted laser desorption and ionization time-offlight mass spectrometry. Antibiotic susceptibility testing was performed by disk diffusion method. E. coli isolates were tested phenotypically for ESBL production using the double disk synergy test and β-lactamase determinants were searched for by PCR and sequencing. The sequence types of the ESBL-producing E. coli isolates were identified by multilocus sequence typing (MLST). Six ESBL-producing E. coli isolates were obtained. All of the isolates were resistant to amoxicillin and cefotaxime, five were resistant to aztreonam and only one was resistant to ciprofloxacin and trimethoprim/sulfamethoxazole. All of the obtained E. coli isolates harbored the blaCTX-M ESBL gene, while only one isolate co-occurred the blaTEM gene. MLST results showed that all strains belonged to the sequence type ST10. This work demonstrated that the migratory white stork (Ciconia ciconia) can play an important role in the dissemination of ESBL-producing E. coli as a potential reservoir and vector.

Biography :

Esma Bendjama has completed her Master’s in Water Microbiology from Annaba University, Algeria. She is a PhD student at Annaba University. She has published 4 papers as author and co-author.