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Feasibility of using multiple locus variable number tandem repeat analysis for epidemiology study of Vibrio parahaemolyticus infections
4th International Congress on Bacteriology and Infectious Diseases
May 16-18, 2016 San Antonio, USA
Min Jiang
Shenzhen Center for Disease Control and Prevention, China
Posters & Accepted Abstracts: J Bacteriol Parasitol
Abstract:
Molecular typing can provide critical information for detecting outbreaks and tracking the source caused by V. parahaemolyticus. In this study, we described the development and evaluation of an optimized multiple locus variable number tandem repeat analysis (MLVA) for characterization of V. parahaemolyticus. The discriminatory power of MLVA was compared with that of PFGE by typing 73 sporadic isolates. Epidemiologic concordance was evaluated by typing 23 isolates from five epidemiologically well-characterized outbreaks. The optimized MLVA were applied in early warning and tracking source of V. parahaemolyticus infections. There was no significant difference in the discriminatory power of PFGE and MLVA with 6 or 8 VNTR loci for sporadic isolates. All isolates within an outbreak were indistinguishable by MLVA with 6 loci with the exception of 3 isolates from one outbreak that had a different type than that of the other 4 isolates from that outbreak. We applied the MLVA with 6 loci to detect two suspected outbreaks. One of suspected outbreaks with same PFGE pattern can be distinguished by MLVA. Although follow-up investigation identified both suspected outbreaks had no epidemiological concordance, MLVA could decrease the frequency of initiating an epidemiological survey. The MLVA type of isolates from one of outbreaks from different districts was the same as that of the shrimp. The data from laboratory and subsequent epidemiological investigation suggested this outbreak may be associated with consumption of shrimps. In conclusion, these results indicated the optimized MLVA may be a promising tool for early warning and epidemiological surveillance of V. parahaemolyticus infections.
Biography :
Email: jiangmin_115@163.com