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Expression of intracellular interferon gamma and perforin in T cells in acquired aplastic anemia patients
5th World Hematologists Congress
August 18-19, 2016 London, UK

Vandana Sharma, Rajiv Kumar, Tulika Seth, Prabin Kumar, Manju Namdeo, Renu Saxena, Uma Kanga and D K Mitra

All India Institute of Medical Sciences, India

Posters & Accepted Abstracts: J Blood Disord Transfus

Abstract:

Aplastic anemia (AA) is characterized by peripheral blood pancytopenia with hypocellular bone marrow involving destruction of hematopoietic stem cells by T cells and their cytokine products. Interferon gamma (IFN-γ) and perforin are one of the mediators of cell destruction. In this study we investigated the expression of intracellular IFN-γ and perforin in T lymphocytes in peripheral blood of 30 acquired aplastic anemia patients by flow cytometry. None of the patients had been started on immune suppressive therapy. The patients were categorized as per disease severity: Non severe AA (NSAA), severe AA (SAA) and very severe AA (VSAA). The mean percentage of CD5 T cells expressing IFN-γ was higher in NSAA as compared to severe and very severe (SAA+VSAA) group (p=0.033) and SAA group (p=0.05). The mean percentage of CD5 T cells expressing perforin was higher in the SAA+VSAA group in comparison to NSAA group (P=0.040). Lower IFN-γ expression in SAA/VSAA cohort as compared to the NSAA cohort could be due to the fact that total T cells available for analysis is much higher in the NSA group as compared to SAA/VSAA. IFN-γ stimulates the effector T cells for activity against the target cell which in case of aplastic anemia is the hematopoietic stem cell. High expression of perforin by CD5 T cells could be a marker of aberrant activation of immune system in response to an as yet unknown inciting agent.

Biography :

Vandana Sharma is currently pursuing PhD under the supervision of Additional Professor Dr. Tulika Seth at the Department of Hematology of All India Institute of Medical Sciences, India.

Email: vsharma1087@gmail.com

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