Journal of Plant Pathology & Microbiology

Expression analysis of Chinese cabbage over-expressed StEREBP subjected to various hormones and stresses

8^th World Congress on Plant Genomics and Plant Science

August 10-11, 2018 Osaka, Japan

Hye-Eun Lee, Jong-Pil Hong, Jinhee Kim, Eun Su Lee and Do-Sun Kim

National Institute of Horticultural and Herbal Science, South Korea

Posters & Accepted Abstracts: J Plant Pathol Microbiol

Abstract:

Ethylene responsive element binding protein (EREBP) is a major group of the AP2/ERF family and plays significant roles in the regulation of abiotic and biotic stress responses. StEREBP is cloned from potato (Solanum tuberosum L.) and characterized to response in abiotic stress and hormone regulation. In this research, we constructed Chinese cabbage StEREBP overexpressing plants using the Agrobacterium-mediated transformation. Six transgenic plants are confirmed the T-DNA insertion by Southern blot analysis and RT-PCR analysis. Phenotype of transgenic plants is not different compared with control plant. The reverse transcription PCR (RT-PCR) showed that cold stress and ABA related genes are increased but ethylene related genes are decreased in StEREBP overexpressing Chinese cabbage plants. The StEREBP responded to abiotic factors and hormones suggested that they possibly had diverse roles in stress and hormone regulation of Chinese cabbage.

Biography :

Hye-Eun Lee is conducting the research on the development of molecular marker in various vegetables. I work at the National Horticultural Science Institute in South Korea, and now I study about analysis of gene expression using Chinese cabbage and construction of genetic population and linkage genetic map related to agronomic traits in onion.

E-mail: helee72@korea.kr