Health Care : Current Reviews

Evaluation of fertility regulating effects of herbal silver nanoparticles to develop a male contraceptive: A novel approach

35^th Global Healthcare Summit

January 27-28, 2025 Webinar

Pallavi Shah

University of Rajasthan, India

Scientific Tracks Abstracts: Health Care Curr Re

Abstract:

Since ancient times plants have been used traditionally for pharmacological and therapeutics. Objectives: Unwanted pregnancies caused population explosion, resulted decreased natural resources and environmental pollution. Fertility control in human is the only measure to check population and provides basic needs to inhabitant on earth planet. Materials and Methods: Colony-bred, healthy adult male albino rats (Rattus norvegicus) were used. Methanolic extracts of prepared and silver nanoparticles of plants Citrullus colocynthis, Moringa oleifera, Pongamia pinnata Putranjiva roxburghii and Tecomella undulata synthesized, characterized, and orally administered in male Wistar rats at different doses for a period of 60 days to evaluate effects on the reproductive organs and functions at the aiming to develop a safe, orally effective, and reversible fertility regulating agent which could be used as male contraceptive. Were prepared and administered orally. CPCSEA and IAEC committee guidelines were followed. Body weight. All animals’ body and organs weights and weights of testes and reproductive accessory organs of all treated animals were recorded. Sperm motility, density and fertility were measured hematological indices and serum clinical investigations were done to assess adverse effects if any caused in treated rats. Proteins, cholesterol, glycogen, ascorbic acid, sialic acid contents in different organs and fructose level in seminal vesicles and FSH, LH and Testosterone levels were measured. For histopathological observations testes and vital organs were fixed in Bouin’s fluid, dehydrated, and stained with Hematoxylin and Eosin. Results: Results of the present study reveals that of herbal AgNPs administration in rats significantly reduced reproductive organ weight and fertility potential. Decreased sperm motility and sperm density, protein, fructose and sialic acid contents and testosterone levels in rats followed nanoparticle treatment indicates adverse effects on reproductive organs and degenerative changes in testicular histopathology and number of spermatocytes, spermatids, and spermatozoa in lumen of seminiferous tubules reduced support androgenic deprivation effects suggests a potential candidate for the development of herbal male contraceptives agent. Conclusion: Androgen suppression caused by silver nanoparticles (AgNPs) treatment inhibit spermatogenic process, reduced sperms motility and density resulted declined fertility possibly due to androgenic nature in rats following treatment as compared to treated vehicles controls. Authors acknowledged to CSIR, UGC, MHRD, RUSA2.O Project No.5 New Delhi, for financial support, Head of the Department of Zoology, University of Rajasthan, Jaipur, India for providing infrastructure facilities. Keywords: Contraception, Fertility, Sperm Motility, Sperm Density, AgNPs, Rat.

Biography :

Pallavi Shah is working as Professor at University of Rajasthan, Jaipur since May 2012; She did M Sc in Zoology and PhD in Reproductive Physiology. She is an active Member of (i) ISCA (ii) ISSRF (iii) SRBCE, associated with the 12 Journals. She has been participated in more than 65 Seminars Symposium/Conferences/Workshops in India and abroad. She organized ICMADRH - 2010, NWBIT- 2015 and ICANTEAGH 2023. She published more than 100 research papers in the journals of international repute; seven research scholars have been awarded PhD under his supervision and 5 are currently working. She visited the USA, Germany, Austria, China, Malaysia, and Thailand to deliver talks. Principal Investigator of MHRD, RUSA 2.0 Project number 5 “Nanoparticles in Healthcare, Sustainable Agriculture and waste water Management”.