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International Conference on Oral Health and Dentistry

Kuala Lumpur, Malaysia
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Evaluation of antibody titer against Porphyromonas gingivalis in the experimental periodontitis induced by oral inoculation of P. gingivalis in the rat model
19th American Dental Congress
December 08-10, 2016 Phoenix, USA

Hedayatipanah M, Torkzaban P, Zamani A, Yousefi M R, Faradmal J and Karimitabar Z

Hamadan University of Medical Sciences, Iran

Posters & Accepted Abstracts: Dentistry


Background: Periodontal disease is results from a complex interaction between the sub-gingival biofilm and the host immuneinflammatory events that it develops in the gingival and periodontal tissues in response to the challenge presented by the bacteria. This condition is associated with the Gram-negative pathogen in the sub-gingival plaque and specific microorganisms such as red complex (Porphyromonas gingivalis, Tannerella forsythia, Treponema denticola). Porphyromonas gingivalis (pg) is one of the wellknown biofilm bacteria, which is associated with human chronic periodontitis. Therefore, in this study, we investigated the adaptive immunity response to whole cell P. gingivalis in the rat model. Objectives: Determination of the antibody titer against the P. gingivalis bacteria in experimental periodontitis in the rat model. Materials & Methods: In this experimental study, we immunized the six groups of eleven, black pigmented pathogen free male rats with either formalin killed whole cells P. gingivalis (FKWC) ATCC 33277 with incomplete Freund�??s adjuvant (IFA), incomplete Freund�??s adjuvant(IFA) with alone PG buffer and non-immunized positive and negative control groups. Result: The data analysis showed that; there was significant difference in the antibody titer (IgG and IgA), between the groups that immunized with FKWC, and the other groups (p<0.001) at one week after the booster injection and two weeks after the lasted inoculation. The bacterial rate was significantly different in the sub-gingival plaque sample of groups 1, 2, 4 and 6 with groups 3 (2200±635.61) and 5 (2650±699.60), but there was no significant difference between groups 1 and 2; that received FKWC vaccine and inoculated, and which were not inoculated with live bacteria respectively (p>0.05). Conclusion: Immunization with formalin killed whole cells P. gingivalis ATCC 33277 which caused the increase of IgG and IgA antibody titer against P. gingivalis bacteria, and it restricted the sub-gingival colonization of live P. gingivalis ATCC 33277 cells.

Biography :

Email: dr.hedayatipanah@yahoo.com