Journal of Bacteriology & Parasitology

Encapsulation of hybridoma cell culture for the higher secretion of anti-A blood grouping mAbs

9^th Biotechnology Congress

August 31-September 02, 2015 Orlando,Florida, USA

Asokan C and O A Catherine

Sokoto State University, Nigeria

Scientific Tracks Abstracts: J Biotechnol Biomater

Abstract:

Biopolymer membrane was prepared by using oppositely charged natural biopolymer as a polymer beads. The biopolymer membrane was used for the encapsulation of three hybridoma cell lines to produce monoclonal antibodies. The morphologies of beads and cells growing were observed in inverted microscope. Cell viability was measured by trypan blue dye exclusion method using a haemocytometer. The increased secretion of monoclonal antibody is confirmed by the antibody by titre analysis and protein estimation. These results indicate the effectiveness of encapsulation for hybridoma cell culture. The present study concludes that the yield of diagnostic antibodies is maximized by the encapsulation method.

Biography :

Asokan C has completed his PhD at the age of 27 years from University of Madras and Postdoctoral studies from Columbia University, USA. He is the Associate Professor, Department of Biochemistry, Sokoto State University, Nigeria. He has published more than 36 papers in reputed journals and has been serving as an Editorial Board Member of reputed journals

Email: asokan_74@hotmail.com