Journal of Vaccines & Vaccination

Efficient induction of T cell activation by CH401MAP peptide; Her2 (N:163-182) in vitro

2^nd International Conference on Vaccines and Vaccination

August 20-22, 2012 Hilton Chicago/Northbrook, USA

Banri Tsuda

Scientific Tracks Abstracts: J Vaccines Vaccin

Abstract:

P eptide vaccine is an attractive approach to induce long-lasting antitumor effecter cells for the control of breast cancer progression. While the efficacy depends on patients� HLA type, the selection of epitope peptide according to the binding motif is not always successful. We found an anticancer effect of CH401MAP peptide that has B cell epitope of Her2 by immunizing the peptide to the mice transplanted with a Her2-positive tumor cells. This peptide also possesses multiple T cell epitopes with moderate affinities to HLA I and II molecules covering nearly 80% of Japanese breast cancer patients as long as SYFPEITHI score was estimated. Hence we tried to evaluate the anticancer effect of the peptide on breast cancer patients. We stimulated 140 PBMC from Japanese breast cancer patients with CH401MAP peptide and IL2 every 7days and checked the cellularity, proliferation of T cells and cytokine production after 21days. As a result, lymphocyte proliferation was observed in 64 of 124cases. The cell proliferation andIL-2 production tend to correlate with Her2 expression level. On the other hand, the cellularity did not significantly change between Her2 negative and positive patients. HLA type had some restriction for the response. These results demonstrate that the peptide induced T cell activation in PBMC of Japanese breast cancer patients with high frequency.

Biography :

1997-2003 Tokai University School of Medicine, Kanagawa; 2003-2005 Resident, Tokai University Hospital, Kanagawa; 2005-2006 Resident, Department of General Surgery, National Hospital Organization Tokyo Medical Center, Tokyo; 2006-2008 Staff Surgeon, Department of Surgery, Tokai University Hospital, Kanagawa; 2008-2009 Staff Surgeon, Department of Surgery, National Hospital Organization Murayama Medical Center, Tokyo; 2009-2012 Reserch Associate, Department of Breast and Endocrine Surgery, Tokai University Hospital, Kanagawa