Journal of Pharmacovigilance

Effect of pretreatment with Polyalthia longifolia var (Annonaceae) on doxorubicin induced cardiotoxicity in rats

2^nd International Conference and Exhibition on Pharmacovigilance & Clinical Trials

November 18-19, 2013 Hilton San Antonio Airport, TX, USA

Ramesh Alluri, Rajashekar Perusomula1 and Kashivishwanath Routhu

Accepted Abstracts: J Pharmacovigilance

Abstract:

Objective: The objective of present study was to investigate the cardio protective effect of Polyalthia longifolia var (Annonaceae) (PL) against the cardio toxicity of doxorubicin (Dox) in rats. Methods: Male Wistar rats (200-250 g) were randomly divided into four groups. Group I- was control, group II- Dox (10 mg/ kg i.v), groups III PL (300 mg/kg orally for 18 days) + (Dox (10 mg/kg i.v) and IV- PL (600 mg/kg orally for 18 days) + (Dox (10 mg/kg i.v). Dox (10 mg/kg i.v) was administered in group II, III and IV on day 16. After anesthetizing the animals on the 18th day electrocardiogram (ECG) was recorded and blood was investigated for creatine kinase-MB is enzyme (CK-MB), lactate dehydrogenase (LDH) and aspartate aminotransferase (AST) while determination of superoxide dismutase (SOD), reduced glutathione (GSH), lipid per oxidation (LPO) and histopathology was carried out for heart. Results: Group 3 and 4 of animals showed decreased QT interval (p<0.01) and was non significant in ST interval and heart rate compared to group 2. Significant decrease in serum CK-MB and AST of group 3 (p<0.01) and group 4 (p<0.001) of animals was observed as compared to group II. In group 4 significant increase in the level of GSH (p<0.01) and decrease in MDA content (p<0.01) was observed as compared to group 2, whereas SOD was non-significant. Histopathological study 600 mg/kg treated group showed protection against myocardial toxicity induced by doxorubicin. Conclusion: It is concluded that Polyalthia longifolia Var possessed cardio protective effect at dose of 600 mg/kg against doxorubicin induced cardio toxicity in rats. Key Words: Antioxidant, electrocardiogram, histopathology, cardiotoxicity, tissue parameters