Journal of Bacteriology & Parasitology

Effect of glutathione on the pyocyanin production in Pseudomonas aeruginosa

International Congress on Bacteriology & Infectious Diseases

November 20-22, 2013 DoubleTree by Hilton Baltimore-BWI Airport, MD, USA

Yani Zhang and Shihong Wang

Accepted Abstracts: J Bacteriol Parasitol

Abstract:

Pyocyanin secreted by Pseudomonas aeruginosa is a redox active virulence factor, generating superoxide and H2O2 in host cells. Glutathione (GSH) is the most effective antioxidant for removing these reactive oxygen species in cells. However, many pulmonary diseases such as cystic fibrosis (CF) are associated with the lower levels of GSH in the epithelial lining fluid (ELF) than those in healthy individuals. Oxidative injury inflicted by P. aeruginosa, in CF lungs is one of the main causes to aggravating disease. We hypothesized that GSH plays an important role during P. aeruginosa mediated pathogenesis. To test this hypothesis, we constructed a glutathione-null gshB mutant. The further result demonstrated that the gshB mutant has less pyocyanin than the wild type PAO1 (P<0.01), while P. aeruginosa strains treated with GSH increased pyocyanin levels. This demonstrated GSH can activate the pyocyanin production; the results indicated that the expression of the gshB and gor gene was downregulated by rhl system. These findings may bring new insights into the molecular pathogenesis of P. aeruginosa infections and lead to novel therapeutic intervention for inhibiting P. aeruginosa infections.

Biography :

Yani Zhang is a teacher of Northwest University in China. Dr. Zhang?s knowledge in the free radical mechanism of water soluble glutathione?s role in maintaining the antibiotic antimycin A effectiveness to resist bacteria Pseudomonas aeruginosa infection is undisputable wealth.