Journal of Bacteriology & Parasitology

E-BABE: Exploration of real-time fluorescence loop-mediated isothermal amplification (LAMP) assay for detection of Isospora suis oocysts

International Conference on Parasitology

August 24-26, 2015 Philadelphia, USA

Cuiqin Huang, Fuli Wen, Liangping Yue, Renfeng Chen, Wei Zhou, LingyingHu, Meizhen Chen and Shoukun Wang

Longyan University,China Fujian Agriculture and Forestry University, China

Posters-Accepted Abstracts: J Bacteriol Parasitol

Abstract:

Isospora suis is an intestinal protozoan parasite in pigs. The 2-3 weeks old piglets are most often infected by I. suisbecause their immune system is not fully developed. The infection exhibits clinical features such as diarrhea and dehydration and seriously affects the economic interests of farmers. The traditional method of identifying I. suis relies on the detection of fecal oocysts, which depends heavily on the accumulation of experience.Thus,missed detection, and false alarms often occur during detection. With the development of molecular-based detection methods, development of a simple, convenient and more sensitive method for the detection of I. suisis an urgent need. In this study, based on the 18S rRNA gene sequence, a real-time fluorescence loop-mediated isothermal amplification (LAMP) assay was established for the detection of I. suis. The results showed that the assay is highly specific and sensitive, with a detection limit of 2.74�?102 copies/μL recombinant plasmid of I. suis, corresponding to 1fg/μL plasmid whenconverted to DNA concentration. The sensitivity is about 100 times higher than conventional PCR. Additionally, DNA extracted froma certain number of oocysts was used for detection, and it showed that the LAMP assay had a detection limit of 5 oocysts, lower than that of 13 oocysts of conventional PCR. The established LAMP assay overcomes the shortage of the traditional microscopy-based method, and provides a valuable way for molecular detection of I. suis.

Biography :

Cuiqin Huang has completed her PhD at the age of 29 years from South China Agricultural University in China. She is a associated professor in Longyan Univerity in China.She has published more than 25 papers in reputed journals and has been serving as an editorial board member of repute.

Email: ami_hagag@yahoo.com