Journal of Bacteriology & Parasitology

Drug susceptibility of Trichomonas vaginalis to Metronidazole in Bushehr city (In Vitro)

10^th International Confernce on Parasitology, Microbiology and Infection Control

November 08-09, 2022 | Webinar

Marzieh Taherzadeh

Microbiology PhD, Bushehr University of Medical Sciences, Bushehr, Iran

Scientific Tracks Abstracts: J Bacteriol Parasitol

Abstract:

Statement of the Problem: Trichomoniasis is the most common non-viral sexually transmitted disease caused by the protozoan parasite Trichomonas vaginalis. Metronidazole (MTZ), a 5-nitroimidazole derivative, is the main antitrichomonal agent of choice for treatment of trichomoniasis. Since 1962, some cases of treatment failure with metronidazole have been reported and increased resistance of the parasite to MTZ has emerged as a highly problematic public health issue. This study was aimed to determine current susceptibility of isolates of T. vaginalis to metronidazole in Bushehr city. Methodology & Theoretical Orientation: This study was performed on 20 T. vaginalis isolates collected from comprehensive health service centers and hospitals of Bushehr city. After axenisation of the parasites, susceptibility testing was carried out by using serial two fold dilutions of MTZ (2 to 64 μg/ml). The minimum inhibitory concentration (MIC) and the minimum lethal concentration (MLC) of the trichomonads were determined after 24 and 48 h incubation at 36 °C. Drug susceptibility assays of the all isolates were carried out two times in triplicate under aerobic and anaerobic conditions. Results: One hundred percent of the T. vaginalis isolates (20/20) were sensitive to MTZ. Resistance to metronidazole was defined as aerobic MIC ≥50 μg/ml, detected in none of isolate. The means of aerobic MICs and MLCs and that of anaerobic MICs of the parasites were 3.8, 3.3 and 2.1 μg/ml, respectively. Conclusion & Significance: Investigation about drug sensitivity in T. vaginalis isolates was done for the first time in Bushehr city. Since no metronidazole-resistant isolate was found, the situation was very promising. However, the need for larger-scale research is inevitable in the province. Considering that the most results are based on the laboratory observations, in vivo study is recommended too. Such studies will lead to promote the development of real standards about clinical and microbiological response to MTZ treatment.

Biography :

Marzieh Taherzadeh works at the Department of Microbiology and Parasitology of Bushehr University of Medical Sciences. Marzieh has her expertise in cell culture, drug discovery and diagnosis of parasite. She also typed the parasite Trichomonas vaginalis in the simultaneous research. Her research has led to the publication of several articles. She has experience in teaching and research in the field of Microbiology & parasitology.