Diversity, bioactivity, and secondary metabolite profiles of bacteria isolated from different tissue of the mollusk gastropod Truncatella species
3rd International Congress on Bacteriology and Infectious Diseases
August 04-06, 2015 Valencia, Spain

Malem Flores1, Mark Jeremiah Cleofas1, Jortan Tun1, Pamela David-Dobay2, Joyce Ibana1 and Gisela Concepcion1

Scientific Tracks Abstracts: J Bacteriol Parasitol


The emergence of drug resistance for many diseases has been for decades the driving force behind the pursuit of naturallyoccurring
bioactive compounds. Among the candidate drug leads, marine sources are one of the most promising owing
to the vast biodiversity of marine organisms. However, marine gastropods with shells are an overlooked source of bioactive
secondary metabolites because they are well-protected by their shell covering and some produce venoms. In this study, several
bacteria were isolated from different tissues of Truncatella sp., a small gastropod mollusk that is found on land very close
to seawater (splash zone). Identification of each isolate using 16S sequence analysis revealed a diversity of Gram-negative
and Gram-positive bacteria, with Actinobacteria as the dominating group. Furthermore, secondary metabolites produced
by Truncatella-associated bacteria showed promising antimicrobial and anti-cancer properties. Some of these bioactive
metabolites manifested narrow spectrum antimicrobial activity against Staphylococcus aureus or Pseudomonas aeruginosa
while having weak inhibitory effect on lactic acid bacteria commensals. Likewise, there were extracts that exhibited selective
cytotoxic activity on the lung cancer cell line, A549 or colorectal cancer cell, HCT 116 with moderate or no cytotoxicity on the
normal kidney cell line, MDCK. Chemical profiles of secondary metabolites determined by reverse-phase high performance
liquid chromatography (RP-HPLC) demonstrated common and unique patterns of metabolites for each isolate. This suggests
that Truncatella is a potential source of bioactive metabolite-producing bacteria which can be valuable for drug discovery and
that secondary metabolite profiles may present distinct phenotypic traits of the microorganisms. Importantly, this is the first
study to report on the genetic and chemical profile diversity of bacteria associated with Truncatella and their corresponding
antimicrobial and anticancer properties.

Biography :

Malem Flores currently working at the Marine Science Institute, Velasquez St. University of the Philippines, Philippines.