Journal of Microbial & Biochemical Technology

Development of molecular kit to minimize the Ciprofloxacin and Amoxyclav resistance strains from chronic supportive otitis media

3^rd World Congress and Expo on Applied Microbiology

November 07-09, 2016 Dubai, UAE

Mahesh Chandra Sahu and Santosh Kumar Swain

Siksha â??Oâ?? Anusandhan University, India

Posters & Accepted Abstracts: J Microb Biochem Technol

Abstract:

Chronic suppurative otitis media (CSOM) is defined as chronic inflammation of middle ear cleft characterized by persistent or intermittent infected ear discharge from a non-intact perforated tympanic membrane at least for 3 months of duration. Commonly ciprofloxacin and amoxyclav are used as safe and popular antibiotics for CSOM. But unfortunately the antibiotics become resistant due to the over use or miss use. For that culture sensitivity is highly required. The sensitivity of bacterial detection in middle ear infections has been improved by PCR. It is useful for the detection of pathogens that are slowly growing, difficult to culture or hazardous to handle in a diagnostic lab. In briefly, the culture sensitivity method is a time consuming and required at least 72 hour to give the report. But in the polymerase chain reaction, we can understand the resistance of ciprofloxacin and amoxyclav with in 3 hour. After getting the result, the clinician may or may not prescribe these antibiotics. The objective of this study is to develop a molecular kit (primers) to early detection of the efficacy of these antibiotics (ciprofloxacin and amoxiclave) with the clinic-microbiological profile of CSOM and to analyze the susceptibility pattern of the aerobic bacterial isolates, so that an antibiotic policy can be formulated for CSOM, for better patient management.

Biography :

Email: mchsahu@gmail.com