Journal of Vaccines & Vaccination

Development immunogen for protection against PRRSV infection using Korean isolates ORF5 protein expression using baculovirus expression system

10^th Euro Global Summit and Expo on Vaccines & Vaccination

June 16-18, 2016 Rome, Italy

Sun Shin Yi, Hye Kyung Baek, Ji Ae Song and Tae-Wook Hahn

Soonchunhyang University, South Korea
Kangwon National University, South Korea

Posters & Accepted Abstracts: J Vaccines Vaccin

Abstract:

Porcine reproductive and respiratory syndrome virus (PRRSV) is the causative agent of reproductive failure and respiratory disorders in porcine industry. The viral genome consists of eight overlapping open reading frames (ORFs). ORF5 encodes one of the major glycoproteins and is known as an immunologically important structural protein associated with virus neutralization. The ORF5 gene of the Korean PRRSV isolate, CNV-1, was amplified by reverse transcription-polymerase chain reaction (RT-PCR), cloned and sequenced. The nucleotide and amino acid sequences of CNV-1 ORF5 shared 91% and 83% identity, respectively with the American isolate (VR2332 strain) and 57% and 49% identity with the European isolate. For the expression and easy purification of ORF5, the cDNA containing the complete ORF5 sequence selected and fused in frame with sequence encoding glutathione S-transferase (GST) was cloned into a baculovirus transfer vector and transfected into Sf9 cells. The GST-ORF5 fusion protein produced in Sf9 cells was detected by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western blotting. Sequencing results confirmed that the recombinant baculovirus from Sf9 cells contains the complete ORF5 gene. Further studies in this direction will address whether ORF5 can be a good candidate for a subunit vaccine against PRRSV in Korea and it should be validated safety before using in the field.

Biography :

Sun Shin Yi has completed his PhD from Seoul National University, South Korea and Postdoctoral studies from Marquette University, USA. Currently he is a Professor in the Department of Biomedical Laboratory Science and an Associate Dean of Special Affairs for Planning and Chair of IACUC in the Soonchunhyang University. He has published more than 50 papers in reputed journals and he is a Board Member of Korea Mouse Phenotype Center (KMPC).

Email: admiral96@gmail.com