Scientific Tracks Abstracts: J Vaccines Vaccin
Rational design of antigen purification and formulation with adjuvants requires a thorough knowledge of intermolecular
forces that affect their structural and colloidal stability. The biophysical techniques used to assess these forces and
interactions constantly evolve, and often require custom modifications to achieve practical goals while conforming to the
theoretical rigor. Examples of adapting existing theories to gain specific insights on vaccine pre- and post-injection stability will
be given. The techniques adapted will include static and dynamic light scattering, electrophoretic mobility, near-UV derivative
spectroscopy, variable speed analytical ultracentrifugation, differential scanning calorimetry and attenuated total reflectance
Fourier-transform infrared spectroscopy. A case study where such expanded analytical techniques were used will be based on
human papillomavirus virus-like-particle (VLP) development.
Henryk Mach is a senior investigator in Bioprocess Analytical and Formulation Sciences, Bioprocess Research and Development at Merck Research Laboratories,
Merck & Co., focusing on applications of biophysical methods in vaccine development. He is an author of over 40 professional papers, book chapters and patents
that reflect his research interests in the spectroscopy, virus assembly phenomena, and computational methods. Before joining vaccine development area at Merck
in 1990, he was a Research Associate in Prof. Middaugh’s laboratory at the University of Wyoming. He received an MS degree in Biotechnology from Agricultural
University and PhD in Biophysics from Jagiellonian University, both in Krakow, Poland.