Journal of Bioequivalence & Bioavailability

Determination of doxycycline in human plasma by liquid chromatography tandem mass spectrometry

3^rd World Congress Bioavailability & Bioequivalence

March 26-28, 2012 Marriott Hotel & Convention Centre, Hyderabad, India

Chaitanya Krishna Atmakuri

Posters: J Bioequiv Availab

Abstract:

A simple, rapid, specific and sensitive liquid chromatography tandem mass spectrometric method has been developed and validated for the estimation of Doxycycline from 150μL of human plasma. Doxycycline is extracted from human plasma by Solid Phase Extraction. Demiclocycline was used as an internal standard. Detection was performed using TSQ Quantum Discovery max mass spectrometer. Chromatographic separation of analytes and internal standard were carried out using a reverse phase C18, column at 500μL flow. The assay of Doxycycline is linear over the range of 0.055μg /mL to 7.612μg/mL with a precision <14.83% and the limit of quantification in plasma for Doxycycline was 0.055μg/mL. Mean extraction recovery obtained was 95.55%.Samples are stable at room temperature for 6 hrs, processed samples were stable at least for 30.20 hrs and also stable at three freeze?thaw cycles. The method has been used to perform pharmacokinetic and bioequivalence studies in humans